Intracellular distribution of nontargeted quantum dots - Supplementary Video: 39658
Supplementary video of original research "Intracellular distribution of nontargeted quantum dots after natural uptake and microinjection" to be published in the open acesss International Journal of Nanomedicine by Damalakiene L, Bagdonas S, Karabanovas V, et al. Background: The purpose of this study was to elucidate the mechanism of natural uptake of nonfunctionalized quantum dots in comparison with microinjected quantum dots by focusing on their time-dependent accumulation and intracellular localization in different cell lines. Methods: The accumulation dynamics of nontargeted CdSe/ZnS carboxyl-coated quantum dots (emission peak 625 nm) was analyzed in NIH3T3, MCF-7, and HepG2 cells by applying the methods of confocal and steady-state fluorescence spectroscopy. Intracellular colocalization of the quantum dots was investigated by staining with Lysotracker. Results: The uptake of quantum dots into cells was dramatically reduced at a low temperature (4C), indicating that the process is energy-dependent. The uptake kinetics and imaging of intracellular localization of quantum dots revealed three accumulation stages of carboxyl-coated quantum dots at 37C, ie, a plateau stage, growth stage, and a saturation stage, which comprised four morphological phases: adherence to the cell membrane; formation of granulated clusters spread throughout the cytoplasm; localization of granulated clusters in the perinuclear region; and formation of multivesicular body-like structures and ...
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Intracellular distribution of nontargeted quantum dots - Supplementary Video: 39658 - Video