Study Points To Possible Cause Of, And Treatment For, Non-Familial Parkinson's

Findings implicate two genetic variants that disrupt protein sorting in neurons

Columbia University Medical Center (CUMC) researchers have identified a protein trafficking defect within brain cells that may underlie common non-familial forms of Parkinsons disease. The defect is at a point of convergence for the action of at least three different genes that had been implicated in prior studies of Parkinsons disease. Whereas most molecular studies focus on mutations associated with rare familial forms of the disease, these findings relate directly to the common non-familial form of Parkinsons. The study was published recently in the online edition of the journalNeuron.

The defective pathway is called the retromer pathway, in part because it can guide the reutilization of key molecules by moving them back from the cell surface to internal stores. In this study, defects in the retromer pathway also appear to have profound effects on the cells disposal machinery, which may explain why Parkinsons disease brain cells ultimately accumulate large protein aggregates. The trafficking defects associated with Parkinsons can be reversed by increasing retromer pathway activity, suggesting a possible therapeutic strategy. No current therapies for Parkinsons alter the progression of the disease.

The researchers also found evidence that, even in unaffected individuals who simply carry common genetic variants associated with an increased risk of Parkinsons disease, these molecular changes are at work. This supports the notion that early treatment approaches will be important in tackling Parkinsons disease.

Taken together, the findings suggest that drugs that target the retromer pathway could help prevent or treat Parkinsons, said study leaderAsa Abeliovich, MD, PhD, associate professor of pathology and cell biology and of neurology in theTaub Institute for Research on Alzheimers Disease and the Aging Brainat CUMC.

In recent years, through genome-wide association studies (GWAS), researchers have identified about 10 common genetic variants that appear to have small effects on the risk of non-familial Parkinsons, However, it has been hard to delve deeper into the impact of these variants. When you look at patient brain tissue at autopsy, its usually too late all the critical dopamine neurons are long gone and the damage has been done, said Dr. Abeliovich.

In the current study, Dr. Abeliovich and his CUMC colleagues used an unusually broad array of approaches including analyses of Parkinsons disease-associated genetic variations, patient brain tissue, in vitro tissue culture studies of brain neurons, and fruit fly (Drosophila) models that harbor genetic variants related to those associated with Parkinsons disease.

The researchers found that common variants in two genes previously linked to Parkinsons disease, LRRK2 and RAB7L1, led to an unexpectedly similar impact on human brain tissue. The effects of the variants were found to be highly overlapping, pointing to a common pathway of action. Prominent cellular changes were observed in the retromer pathway, which is involved in the trafficking of proteins from the Golgi apparatus (which packages proteins for delivery to other cell components) to the lysosomes (which recycle proteins and other molecules). Mutations that affect the retromer pathway have also been found in familial Parkinsons disease. Earlier studies CUMCsTaub Institutehave shown that genetic variants in genes associated with retromer function are linked to Alzheimers disease and retromer component levels appear altered in Alzheimers disease brains, suggesting a broader role for retromer dysfunction in neurodegenerative diseases of aging, according to Dr. Abeliovich.

The impact of the RAB7L1 and LRRK2 variants was apparent even in individuals with no signs or symptoms of Parkinsons disease. This suggests that there is a pre-disease state in unaffected carriers of the two genetic variants that favors early disease onset and that, in theory, could be targeted therapeutically.

The CUMC researchers also demonstrated that overexpression of one of the variants, RAB7L1, can overcome the effects of the other variant. Similarly, expression of VPS35, a gene involved in the retromer pathway, can suppress LRRK2 mutant pathology. It will be interesting to look for drugs that directly target these retromer components or that more generally promote flow through the pathway, said Dr. Abeliovich.

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Study Points To Possible Cause Of, And Treatment For, Non-Familial Parkinson's