Mesenchymal Stem Cell – ResearchGate

Lawrence Jyh Liew added an answer: How can I eliminate contamination in my human MSCs culture?

I believe they are mainly yeast infections due to the white film that develops in the wells but even when that is not present I seem to get high cell death when culturing human MSCs on thermanox with PRP.

Will alterations in LINC proteins change response?

Lab studies show how MSC respond to the dynamic physical environment.

We are trying establish a protocol in order to obtain MSC from cord tissue according protocols described in different papers. Although most of bibliography described that is easy procedure, we are having many difficulties. We cant obtain cells using explants method. We already did 17 cord tissue and we cant see any cells. What can be we are doing wrong?

I created a new protocol to derive MSC from human induced pluripotent stem cells. I characterized the cells using anti-CD73 and anti-CD105. I got 80 to 90% positive cells for the two markers CD73/CD105. I wanted to know if this two markers are sufficient to conclude that my cells are MSC?

This is an interesting question! As said before the ISCT defined a list of criteria to define MSC.( http://www.ncbi.nlm.nih.gov/pubmed/16923606) Nowadays this criteria have been discussed because do not give information about cells potency (http://www.nature.com/news/stricter-standards-sought-to-curb-stem-cell-confusion-1.13434) Usually you can identify MSC using: Plastic adherence Flow cytometry: positive for (CD29,CD44, CD73,CD90, C105) and negative markers for hematopoietic cells (CD34, CD45) endothelial cells CD31 and other plastic adherent cells such as macrophages CD14. Differentiation into adipogenic, osteogenic and chondrogenic lineages

Hum Gene Ther Methods. 2013 Feb;24(1):38-48. doi: 10.1089/hgtb.2012.185. Direct head-to-head comparison of cationic liposome-mediated gene delivery to mesenchymal stem/stromal cells of different human sources: a comprehensive study. Boura JS1, Santos FD, Gimble JM, Cardoso CM, Madeira C, Cabral JM, Silva CL.

My lab is currently looking for the above cells, but unfortunately, can't find anyone with these cells.

Hey Ling, Thanks very much for the answer - any chance to drop you an email? faii

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Mesenchymal Stem Cell - ResearchGate