Category Archives: Mesenchymal Stem Cells

Adipogenic differentiation potential of MSCs following 3D culture. Following 7 days of suspension culture, MSCs were recovered from mesenspheres, plated onto TCPS and maintained under growth or adipogenic culture conditions. The adipogenic differentiation potential of recovered cells was compared to that of conventional monolayer cultures. Brightfield (a, b, e, f, i, j) and phase (c, d, g, h) images of Oil Red O-stained MSCs were obtained following differentiation. a, c, e, g, i Lipid vacuole formation was not evident in any cultures maintained under growth conditions. b, d, f, h, j Lipid vacuoles were more widespread within cultures recovered from mesenspheres following 14 days of differentiation than in monolayer cultures after 28 days of differentiation. Scale bar 200 m. k Quantitative staining for adipogenesis exhibited significant differences between monolayer cultures and cells recovered from mesenspheres. Cells recovered from mesenspheres stained more robustly for Oil Red O than monolayers at day 14 of differentiation, with plated spheroids exhibiting a greater than 20-fold increase (*p< 0.0001) and dissociated spheroids exhibiting a greater than 40-fold increase (*p … Continue reading →

Aldehyde Dehydrogenase Activity Of Breast Cancer Stem Cells Markers used to isolate cancer stem cells, such as CD44, CD24, CD133, aldehyde dehydrogenase (ALDH), and Hoechst leading to uncontrolled self-renewal of CSCs.[11. H. Korkaya et al., Breast cancer stem cells, cytokine networks, and the tumor Daily injection with 50 mg/kg sulforaphane for two weeks reduced aldehyde dehydrogenasepositive cells by more than 50 Review Mesenchymal stem cell therapy for attenuation of scar formation during wound healing What Are The Ethical Issues With Using Embryonic Stem Cells Bones Grown In Lab Using Stem Cells Embryonic Stem Cells Without Destroying Embryos theres been a steady march toward making adult cells mimic embryonic stem cells. Success could ultimately defuse the debate over destroying embryos to harvest the stem cells, which have the ability to transform into any type of human cell, and which Stem Cells Simple Almost from the moment he reported his stem cell breakthrough two years ago, Shinya Yamanaka has been the most famous scientist in the world. The Japanese researcher shocked the globe when he announced that he could coax human skin cells to In contrast, with the new technique, a cell of interest can be chosen Embryonic Stem Cells Without Destroying … Continue reading →

Home Stem Cell Therapy | Cellular Prolotherapy Ross Hauser, MD Ross Hauser, MD: the use of Stem Cell Therapy in the treatment of joint and spine degeneration. Stem cell therapy is exploding in the medical field, and for good reason. Stem cells have the potential to regenerate into any type of body tissue. The amazing thing about stem cells is that when you inject them into the body, they know what kinds of cells your body needs for example, meniscus cells or cartilage cells. It is a very exciting time for medicine, especially in the field of regenerative medicine. In our office we often refer to this as Cellular Prolotherapy. In Stem Cell Therapy we use a persons own healing cells from bone marrow, fat, and blood (alone or in various combinations) and inject them straight to the area which has a cellular deficiency. The goal is the same: to stimulate the repair of injured tissues. Stem cells aid in fibroblastic proliferation where cell growth, proteosynthesis, reparation, the remodeling of tissues, and chondrocyte proliferation occurs. Our bone marrow contains stem cells,also termed mesenchymal stem cells and progenitor cells, among other names. These immature cells have the ability to become tissues … Continue reading →

Stem cell research is on the rise, giving hope to patients and providing treatment for many diseases and disorders. While stem cell treatments are a fairly new science, they can have life-saving effects. Stem cell treatments are effective at treating autoimmune diseases, cerebral palsy, degenerative joint disease, multiple sclerosis, osteoarthritis, rheumatoid arthritis, spinal injuries and type 2 diabetes. It is thought that in the future, stem cell treatment can be used to treat Alzheimer's disease. Stem cell therapy can reduce pain and discomfort; it can help patients suffering from arthritis regain mobility. In serious cases, such as cerebral palsy and multiple sclerosis, stem cell treatments can be life-saving. Because stem cell treatment is a new science, little is known about its long term effects. According to Cell Medicine, no side effects have been reported by patients other than pain at the injection site. Sometimes the behaviors stem from a history of abuse or continuous exposure to a ... Several different treatment methods are commonly used... Heat and cold treatment such as ice and heating pads can be alternated for reducing inflammation and pain ... Regenerative Stem Cell... B cells and T cells operate in the adaptive immune response--the immune system's … Continue reading →

Mesenchymal stem cell therapy is one of two types of stem cell therapy that has been under investigation for treatment of multiple sclerosis. The other type is called hematopoietic stem cell therapy, and both have demonstrated varying degrees of benefit for MS patients. Although the two types of stem cell therapy have some significant differences, one of special importance is the fact that patients undergoing hematopoietic stem cell transplantation must first undergo extensive chemotherapy to eliminate an individuals defective immune system. This places patients at significant risk of infection. Mesenchymal stem cell therapy does not require this step. What is mesenchymal stem cell therapy? Mesenchymal stem cells are derived from adult bone marrow, skin and fat tissue, and umbilical cord blood. This therapy is still in the experimental stages and is being studied in multiple sclerosis for their ability to reduce inflammation, support the immune system, and help prevent and repair tissue damage. This therapeutic approach involves extracting mesenchymal stem cells from a patients own bone marrow and expanding them for several week in a specialized culture lab. The cells are frozen and then thawed before they are infused back into the same patient via an intravenous line. In hematopoietic … Continue reading →

Summary of "Mesenchymal Stem Cell Therapy for Inflammatory Bowel Disease: A Systematic Review and Meta-analysis." Recent advances in inflammatory bowel disease (IBD) therapeutics include novel medical, surgical, and endoscopic treatments. Among these, stem cell therapy is still in its infancy, although multiple studies suggest that the immunomodulatory effect of stem cell therapy may reduce inflammation and tissue injury in patients with IBD. This review discusses the novel avenue of stem cell therapy and its potential role in the management of ulcerative colitis and Crohn's disease. We conducted a comprehensive literature search to identify studies examining the role of stem cell therapy (without conditioning and immunomodulatory regimens) in IBD. Taken together, these studies suggest a promising role for stem cell therapy in IBD although the substantial challenges, such as cost and inadequate/incomplete characterization of effect, limit their current use in clinical practice. This article was published in the following journal. Name: Inflammatory bowel diseases ISSN: 1536-4844 Pages: Mesenchymal Stem Cell Transplantation Transfer of MESENCHYMAL STEM CELLS between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). Mesalamine An anti-inflammatory agent, structurally related to the SALICYLATES, which is active in INFLAMMATORY BOWEL DISEASE. It is considered … Continue reading →

Current Research We are currently engaged in optimization of culture conditions of human bone marrow derived mesenchymal (stromal) stem cells for their expansion and differentiation into cartilage forming cells for the future repair of cartilage defects occurring in osteoarthritis and rheumatoid arthritis. A proprietary system is being explored for the delivery of these cells to the precise location of defects. The research is aimed also to demonstrate (via collaborative efforts with another institution) the lack of any cancer promoting ability in these cultured cells. Future Research We intend to optimize the expansion of marrow-derived mesenchymal (stromal) stem cells and to demonstrate their immune modulatory properties to allow future cellular therapy for Inflammatory Bowel Diseases: Ulcerative Colitis and Crohns Disease. Once this goal is reached, research could expand to other immune-related diseases. Funding Needs So far, all research has been funded from internal sources. In the near future, we are interested in private equity funding. In addition, applications for government small business grants are being considered. View post: Stem Cell Sciences, Inc. - Stem Cell Research … Continue reading →

The stem cell controversy is the consideration of the ethics of research involving the development, usage, and destruction of human embryos. Most commonly, this controversy focuses on embryonic stem cells. Not all stem cell research involves the creation, usage and destruction of human embryos. For example, adult stem cells, amniotic stem cells and induced pluripotent stem cells do not involve creating, using or destroying human embryos and thus are minimally, if at all, controversial. The use of stem cells has been happening for decades. In 1998, scientists discovered how to extract stem cells from human embryos. This discovery led to moral ethics questions concerning research involving embryo cells, such as what restrictions should be made on studies using these types of cells? At what point does one consider life to begin? Is it just to destroy an embryo cell if it has the potential to cure countless numbers of patients? Political leaders are debating how to regulate and fund research studies that involve the techniques used to remove the embryo cells. No clear consensus has emerged. Other recent discoveries may extinguish the need for embryonic stem cells.[1] Since stem cells have the ability to differentiate into any type of cell, … Continue reading →

Mesenchymal stem cells (MSCs) are adult stem cells traditionally found in the bone marrow. However, mesenchymal stem cells can also be isolated from other tissues including cord blood, peripheral blood, fallopian tube, and fetal liver and lung. Multipotent stem cells, MSCs differentiate to form adipocytes, cartilage, bone, tendons, muscle, and skin. Mesenchymal stem cells are a distinct entity to the mesenchyme, embryonic connective tissue which is derived from the mesoderm and differentiates to form hematopoietic stem cells. Morphologically, mesenchymal stem cells have long thin cell bodies with a large nucleus. As with other stem cell types, MSCs have a high capacity for self renewal while maintaining multipotency. Thus, mesenchymal stem cells have enormous therapeutic potential for tissue repair. MSCs have been shown to be capable of differentiating into multiple cell types including adipocytes, chondrocytes, osteocytes, and cardiomyocytes. R&D Systems offers products designed to identify the progression of MSCs into osteogenic, adipogenic, muscular, and chondrogenic lineages. Read more from the original source: Mesenchymal Stem Cells Research Area: R&D Systems … Continue reading →

Statement of Benefit to California: Acute lung injury is a common cause of acute respiratory failure in critically ill patients. Based on recent studies, acute lung injury occurs annually in approximately 24,000 patients in California alone with an estimated mortality of 40% in those patients. Acute lung injury can develop in normal individuals who have been injured in major trauma, such as following an automobile accident or from exposure to smoke fumes in a fire. It also can occur in otherwise normal individuals who develop severe pneumonia from a viral infection, such as the flu. Acute lung injury also develops in patients with a variety of chronic medical illnesses including diabetes, chronic lung diseases, liver disease and dialysis dependent kidney failure. Acute lung injury develops in both pediatric and adult patients and treatment is required in an intensive care unit where patients can receive life support with mechanical ventilation and other life saving therapies. In spite of progress in supportive care for this syndrome of acute respiratory failure, 40% of patients with acute lung injury do not survive. Also, the patients who do survive require long periods of hospitalization to recover and also require extended periods of rehabilitation in specialized … Continue reading →