Duke Researchers Show How Stem Cell Mutations Arise and Metastasize in the Colon – Clinical OMICs News

Scientists at the Duke Cancer Institute report that they have observed how stem cell mutations quietly arise and spread throughout a widening field of the colon until they eventually predominate and become a malignancy. Working with mice, the researchers visually tagged colon cancer mutations by causing stem cells to glow. Mutations found in colon cancer were then visualized in the animals, showing how one or another mutation prevailed over the others to become the driving force of a malignancy.

The teams study (A cancer rainbow mouse for visualizing the functional genomics of oncogenic clonal expansion) appears in Nature Communications.

Field cancerization is a premalignant process marked by clones of oncogenic mutations spreading through the epithelium. The timescales of intestinal field cancerization can be variable and the mechanisms driving the rapid spread of oncogenic clones are unknown. Here we use a Cancer rainbow (Crainbow) modeling system for fluorescently barcoding somatic mutations and directly visualizing the clonal expansion and spread of oncogenes. Crainbow shows that mutations of -catenin (Ctnnb1) within the intestinal stem cell results in widespread expansion of oncogenes during perinatal development but not in adults, the investigators wrote.

In contrast, mutations that extrinsically disrupt the stem cell microenvironment can spread in adult intestine without delay. We observe the rapid spread of premalignant clones in Crainbow mice expressing oncogenic Rspondin-3 (RSPO3), which occurs by increasing crypt fission and inhibiting crypt fixation. Crainbow modeling provides insight into how somatic mutations rapidly spread and a plausible mechanism for predetermining the intratumor heterogeneity found in colon cancers.

This study provides new insight into the previously invisible process in which mutant precancerous stem cells spread throughout the colon and seed cancer, said Joshua Snyder, Ph.D., assistant professor in the departments of surgery and cell biology at Duke and corresponding and co-senior author of the article in Nature Communications. Our technique sets a firm foundation for testing new therapies that interrupt this early, pre-malignant process. We hope to one day target and eliminate these stealth precancerous cells to prevent cancer.

Snyder and colleagues, including co-senior author H. Kim Lyerly, M.D., George Barth Geller professor at Duke, applied the molecular dyeing technique in a new way, tagging several common colon cancer mutations in the stem cells of a single tumor to create a fluorescent barcode. When transferred to a mouse, the rainbow of fluorescent stem cells could be visually tracked, revealing the cellular and molecular dynamics of precancerous events. In this way, the researchers found key differences in how the intestinal habitats common to babies and adults grow precancerous fields of mutant cells.

At a critical period, newborns are sensitive to the effects of mutations within intestinal stem cells. This seeds large fields of premalignant mutated cells throughout the intestine (a process called field cancerization) that dramatically increases cancer risk. These fields of mutated cells can grow and spread for years without being detected by current screening technologies; often, they remain harmless, but under proper conditions, can rapidly become cancerous later in adults.

The researchers also observed that some colon cancer mutations found in patients can lead to an increase in the fertility of the environment surrounding precancerous fields. Ultimately, this leads to the rapid spread of fields throughout the intestine, with lethal consequences.

Certain common mutations that arise from external sources, such as an injury or an environmental exposure, could also disrupt the environment surrounding the stem cell and lead to the rapid growth and spread of precancerous fields. These occurrences can be especially lethal in adults and occur much more rapidly than previously expected.

Field cancerization has been suggested to be the defining event that initiates the process of cancer growth, including cancers of the breast, skin, and lung, Snyder noted. Our technique allows us to model how premalignant cells compete and expand within a field by simple fluorescent imaging, potentially leading to earlier diagnosis and treatment.

Snyder said additional studies are underway using the fluorescent barcoding to view the cancer fields in breast cancer, aiming to learn more about when a precancerous condition known as ductal carcinoma in situ is driven by malignant vs. benign mutations.

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Duke Researchers Show How Stem Cell Mutations Arise and Metastasize in the Colon - Clinical OMICs News